期刊目次

加入编委

期刊订阅

添加您的邮件地址以接收即将发行期刊数据:

Open Access Article

Novel Chemical Reactions and Mechanisms. 2024; 1: (1) ; 18-27 ; DOI: 10.12208/j.ncrm.20240004.

Evaluation of Extractive Values, Qualitative and Quantitative Phytochemical Constituents of Red Soko (Celosia Trigyna) and Green Soko (Celosia argentea)
评估红色鸡冠花 (Celosia Trigyna)和绿色鸡冠花 (Celosia argentea)的提取价值、定性和定量植物化学成分

作者: Jacob Olalekan Arawande1 *, Christianah Olusola Ayodele1, Edgar Uzzezzi. Amuho2, Babawale Peter Olatunji3, Ayodeji Temitope Adesuyi4, Bamidele Imoukhuede4

1 Department of Science Laboratory Technology, University of Medical Sciences, PMB 536 Ondo-City, Ondo State, Nigeria

2 Department of Chemistry, Adeyemi College of Education, PMB 520 Ondo City, Ondo State, Nigeria

3 Department of Bioscience and Biotechnology, University of Medical Sciences, PMB 536 Ondo-City, Ondo State, Nigeria

4 Department of Science Laboratory Technology, Rufus Giwa Polytechnic, PMB 1019 Owo, Ondo State, Nigeria

*通讯作者: Jacob Olalekan Arawande,单位: Department of Science Laboratory Technology, University of Medical Sciences, PMB Ondo-City, Ondo State, Nigeria;

发布时间: 2024-11-22 总浏览量: 139

摘要

药用植物是生物活性化合物不可或缺的来源,并已被证明是广泛应用的坚实成分。研究了五种不同溶剂在提取生物活性成分方面的效力;对红色 soko 和绿色 soko 的植物化学物质进行了定性和定量测定。将植物切成小块,风干,磨成粉末样品,用 40 毫米网眼筛分并正确标记。使用五种不同的溶剂(丙酮、氯仿、乙酸乙酯、甲醇和水)以 1:10 的比例提取每个样品 72 小时。对每种溶剂提取物进行九种植物化学物质(黄酮类化合物、胡萝卜素、苯酚、草酸盐、单宁、皂苷、生物碱、植酸盐和抗坏血酸)的筛选。观察到红色和绿色 soko 中的植物提取物都含有七种植物化学物质。红豉和绿豉中植物化学成分的最高提取率及定性筛选均在水和甲醇提取物中进行。植物化学定量分析表明,两种蔬菜中皂苷、植酸和抗坏血酸的含量较高。红豉的抗坏血酸、皂苷、总酚、总胡萝卜素、生物碱和黄酮含量低于绿豉,绿豉的植酸和单宁含量低于红豉。红豉中黄酮、总胡萝卜素和生物碱含量差异不显著(P˂0.05),绿豉中总胡萝卜素和生物碱含量差异不显著(P˂0.05)。

关键词: 植物化学成分;溶剂;萃取值;红色soko和绿色soko

Abstract

Medicinal plants are indispensable sources of bioactive compounds and have proved to be stalwart ingredients for a wide range of applications. The potency of five different solvents in extracting bioactive constituents; qualitative and quantitative determination of phytochemicals of red soko and green soko were studied. The plant were cut into smaller pieces, air-dried, ground into powdery sample, sieved with 40 mm mesh size and properly labelled. Each sample was extracted using five different solvents (acetone, chloroform, ethyl acetate, methanol and water) at ratio 1: 10 for 72 h. Each solvent extract was screened for nine phytochemicals (flavonoid, carotenoid, phenol, oxalate, tannin, saponin, alkaloid, phytate and ascorbic acid). It was observed that the plant extract contained seven phytochemicals in both red and green soko. The highest extractive values and qualitative screening of phytochemicals in red soko and green soko were obtained in water and methanol extracts. Quantitative phytochemical analysis showed that there was higher content of saponin, phytate and ascorbic acid in the two vegetables. Red soko contained lower ascorbic acid, saponin, total phenol, total carotenoid, alkaloid and flavonoid than green soko while green soko had lower phytate and tannin than red soko. There was no significant difference (P˂0.05) in flavonoids, total carotenoid and alkaloid contents in red soko and in green soko there was no significant difference (P˂0.05) in total carotenoid and alkaloid contents.

Key words: Phytochemical; Solvent; Extractive values; Red soko and green soko

参考文献 References

[1] Crozier, A., Borges, G. and Ryan, D. (2010). The glass that cheers: Phenolic and polyphenolic constituents and the beneficial effects of moderate red wine consumption. The Biochemist, 32(6): 4-9. 

[2] Anderson, G. D. (2004). Phytochemical. Dynamic Chiropractic, 2 issue 01. In: Chung, K.T., Weichang, I. and Johnson, M.G. (1998). Are tannins a double edged sword in biology and health? Trends Food Science Technology, 4, 168-175.

[3] Liu, R. H. (2004). Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutrition, 134, 34795-34855.

[4] Chivandi, E., Mukonowenzou, N., Nyakudya, T., and Erlwanger, K.H. (2015). Potential of indigenous fruit-bearing trees to curb malnutrition, improve household food security, income and community health in Sub-Saharan Africa: A review. Food Research International, 76(4), 980-985.

[5] Prior, R.L. and Gu, L. (2005). Occurrence and biological significance of proanthocyanidins in the American diet. Phytochemistry, 66(18), 2264-2280.

[6] Dykes, L. and Rooney, W. (2007). Phenolic compounds in cereal grains and their health benefits. Cereal Foods World, 52, 105-111.

[7] Liu, R. H. (2013). Health-promoting components of fruits and vegetables in the diet. Adv Nutr., 14(3), 384S-92S. doi: 10.3945/an.112.003517.

[8] Oz, A. T. and Kafkas, E. (2017). Phytochemicals in Fruits and Vegetables. Superfood and Functional Food - An Overview of Their Processing and Utilization. IntechOpen, 1, 175-184. DOI: https://doi.org/10.5772/66987.

[9] Ndolo, V.U. and Beta, T. (2014). Comparative studies on composition and distribution of phenolic acids in cereal grain botanical fractions. Cereal Chem, 91(5), 522-530. doi: 10.1094/Cchem-10-13-0225-R.

[10] Uusiku, N. P. Oelofse, A., Duodu, K. G. Bester, M. J., and Faber, M. (2010). Nutritional value of leafy vegetables of sub-Saharan Africa and their potential contribution to human health: a review. Journal of Food Composition and Analysis, 23, 499-509.

[11] Kaur, C. and Kapoor, H. C. (2002). Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology, 37, 153-161.

[12] Banerjee, A., Datta, J. K., Mondal, N. K. (2012). Biochemical changes in leaves of mustard under the influence of different fertilizers and cycocel. Journal of Agricultural Technology, 8(4), 1397-1411.

[13] Chang S. K., Nagendra, P. K., and Amin, I. (2013). Carotenoids retention in leafy vegetables based on cooking methods. International Food Research Journal, 20(1), 457-465.

[14] Williamson, G., Dupont, M. S., Heaney, R. K., Roger, G., and Rhodes, M. J. (1997). Induction of slutathione S transferase activity in hepG2 cells by extracts of fruits and vegetables. J. Food Chem., 2, 157-160.

[15] Elias, K. M., Nelson, K. O., Simon, M., and Johnson, K. (2012). Phytochemical and antioxidant analysis of methanolic extracts of four African indigenous leafy vegetables. Ann Food Sci. Technol., 13(1), 37-42.

[16] Raghavendra, M, Reddy, A. M., Yadav, P. R., Raju, A. S., and Kumar, L. S. (2013). Comparative studies on the in vitro antioxidant properties of methanolic leafy extracts from six edible leafy vegetables of india. Asian J Pharm Clin Res., 6(3), 96-99.

[17] Grubben, G. J. H. and Denton, O. A. (2004). Plant Resources of Tropical Africa 2 (Vegetables). PROTA Foundation, Wageningen, Netherlands, 167-173.

[18] Kai, M. and Thomas, B. (2005). Phylogenetics of Amaranthaceae using matK/trnK sequence data evidence from parsimony, likelihood and Bayesian approaches. Annals of the Missouri Botanical Garden, 66-102.

[19] IITA. (1972). International Institute for Tropical Agriculture: Root, Tuber and Vegetable Improvement Programme Report. Ibadan, Nigeria.

[20] Ilodibia, C. V., Chukwuma, C., Chukwuma, U. M., Akachukwu, E. E., Igboabuchi, N. A., and Adimonyemma, R. N. (2016). Anatomical, proximate, mineral and vitamin studies on Celosia argentea (Linn.). British Biotechnology Journal, 15(4), 1-17. 

[21] Olawuyi, O. J., Bamigbegbin, B. J., and Bello, O. B. (2016). Genetic variation of morphological and yield characters of Celosia argentea L. germplasm. Journal of Basic and Applied Research International, 13(3), 160-169.

[22] Arawande, J. O. and Aderibigbe, A. S. (2020). Stabilization of edible oils with bitter leaf (Vernonia amygdalina) and water bitter leaf (Struchium sparganophora) extracts. SAR Journal of Medical Biochemistry, 1(1), 9-15.

[23] Arawande, J. O., Akinnusotu, A., and Alademeyin, J. O. (2018). Extractive value and phytochemical screening of ginger (Zingiber officinale) and turmeric (Curcuma longa) using different solvents. International Journal of Traditional and Natural Medicine, 8(1), 13-22.

[24] Bopitiya, D. and Madhujith, T. (2014). Efficacy of pomegranate (Punica granatum L.) peel extracts in suppressing oxidation of white coconut oil used for deep frying. Tropical Agricultural Research, 25(3), 298-306.

[25] Iqbal S. and Bhanger, M. I. (2006). Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. Journal of Food Composition and Analysis, 19, 544-551.

[26] StankoviC, M. S., Zia-Ul-Haq, M., BojoviC, B. M., and UZoviC, M. D. (2014). Total phenolics, flavonoid content and antioxidant power of leaf, flower and fruits from cornelian cherry (Cornus mas l.). Bulg. J. Agric. Sci., 20, 358-363.

[27] Sofowora, A. (2008). Medicinal plants and traditional Medicine in Africa, 3rd ed. Spectrum Blocks Limited, Ibadan, Nigeria, 199-204.

[28] Hunds, S.S., Prakash, P., and Roy, B. (1985). Bioactivity directed extraction and fractionation of E. alba: An hepatoprotective drug of Indian Origin. Ind. J. Pharm. Sci., 13, 50-51.

[29] Joshi, A., Bhobe, M., and Saatarkar, A. (2013). Phytochemical investigation of the roots of Grewia microcos. J. Chem. Pharm. Res., 5: 80-87.

[30] Brindha, S. B. and Purushothaman, K. (1981). Phytochemical analysis of E. alba. BMEMR, 3(1): 84-96.

[31] AOCS. (2004). Official Methods and Recommended Practices of American Oil Chemists Society, 5th Ed. Champaign, IIinoise: American Oil Chemist’s society, USA.

[32] AMC-RSC. (2002). Methods of Analysis of Analytical Methods Committee of Royal Society of Chemistry AMC-RSC 222-239.

[33] Onwuka, G. I. (2005). Food Analysis and Instrumentation: Theory and practice. Naphthali prints, Lagos, Nigeria, 134-138.

[34] Brunner, J. H. (1984). Direct Spectrophotometric Determination of saponin. Anal. Chem., 34, 1314-1326.

[35] Harborne, J.B. (1973). Phytochemical Methods 3rd ed. Chapman and Hall Ltd., 135-203.

[36] Maga, J. A. (1983). Phytates: Its Chemistry, Occurrence, Food Interaction, Nutritional Significance and Method of Analysis. Anal. Chem., 33, 1005-1020. 

[37] MCMFA. (1982). Manual of Chemical Methods of Food Analysis: Food and Drugs Administration and Laboratory Services, Federal Ministry of Health, Lagos, 1-90.

[38] Arawande, J. O., Adeleke, R. Orimoloye, R. O., Adebisi, S. A., Amuho, E. U. (2021). Extractive Values and Antioxidant Properties of Leaves, Seeds, Pods and Coats Moringa Plant. Biomed J. Sci & Tech Res, 39(4). BJSTR.MS.ID.006334.

[39] Ramamurthy, V. and Sathiyadevi M. (2017). Preliminary phytochemical screening of methanol extract of Indigofera trita linn. Journal of molecular Histology &Medical Physiology, 1-11.

[40] Egharevba, H. O. and Kunle, F. O. (2010). Preliminary phytochemical and proximate analysis of the leaves of Piliostigma thonningii (schumach) Milne- Redhead Ethnobotanical Leaveslets, 14, 570-577.

[41] Forman, M.R. and Lanza, L.C. (1993). The correlation between two dietary assessments of carotenoid food composition data base. Am. J. Clin. Nutr., 58, 519-524.

[42] Oboh, G. (2005). Effect of blanching on the antioxidant properties of some tropical leafy vegetables LWT, 38, 513-517.

[43] Amic, B., Aburjai, T., and Al-Khalil, O. (2002). Antioxidative and radical scavenging effects of olive cake extract. Fitoterapia, 73, 456-461. 

[44] Osagie, A. U. (1998), Antinutritional factors in nutritional quality of plantfoods. Editors:osagie, A.U. and Eka, O.U., publisher: Post Harvest Research unit, University of Benin, Benin City, 221-244.

[45] Evans, W.C. (2002). Trease and Evans pharmacognosy, 11th edition, Elsevier, India, 289-291.

[46] Ngbede, J., Yakubu, R. A. and Nyam, D. A. (2008). Phytochemical screening for active compounds in Canarium Scheinfurthii (Atile) leaves from Jos North, Plateau State, Nigeria. Medwell Research Journal of Biological Science, 3(9), 1077-1078.

[47] Seigler, D.S. (1998). Plants with saponins and cardiac glycosides www.life.vinc.edu/plantbio/363/saponinslides.

[48] Ayitey-Smith, E. (1989). Properties and scope: Plant medicine in health care. Ghana University Press, Accra, 29.

[49] Grill, L.S. (1992). Ethnobotanical uses of plants in Nigeria. University of Benin press, Benin City.

[50] Obasi, N. L., Egbuonu, A. C. C., Ukoha, P. O., and Ejikeme, P. M. (2011). Comparative phytochemical and antimicrobial screening of some solvent extracts of Smanaea saman (fabaceae or Mimosaceae). In proceeding of Chemical Society of Nigeria, 34th Annual International Conference, Workshop and Exhibition held at Main Auditorium, University of Ilorin on 19th-23rd Sept., ppORG037-ORG043.

[51] Enwere, N. J. (1998). Foods of plant Origin. 1st edition. Afroobis Publication Limited, Nsukka, Nigeria, 78-85.

[52] Kadler, B. and Boot, H. (2007). Collagens at glance. Journal of Cell Science, 120: 1955-1958.

[53] Achikanu, C. E., Eze-Steven, P. E. Ude, C. M., and Ugwuokolie, O. C. (2013). Determination of the vitamin and mineral composition of common leafy vegetables in south eastern Nigeria. International Journal of Current Microbiology and Applied Sciences, 2, 347-353.

[54] Fasuyi, A. O. (2006). Nutritional potentials of some tropical vegetables meals. Chemical characterization and functional properties. African journal of Biotechnology, 5, 49-53.

[55] Mensah, J. K., Okoli, R. I., Ohaju-Obodo, J. O., and Eifediyi, K. (2008). Phytochemical nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. African Journal of Biotechnology, 7(14), 2304-2309.

引用本文

JacobOlalekanArawande, ChristianahOlusolaAyodele, EdgarUzzezzi.Amuho, BabawalePeterOlatunji, AyodejiTemitopeAdesuyi, BamideleImoukhuede, 评估红色鸡冠花 (Celosia Trigyna)和绿色鸡冠花 (Celosia argentea)的提取价值、定性和定量植物化学成分[J]. 新型化学反应与机制, 2024; 1: (1) : 18-27.